library(rmodel) print(package.version("rmodel")) print(package.version("mouse4302rmodelcdf")) #read in the CEL data mydata<-ReadAffy(cdfname="mouse4302rmodelcdf") ############################################################################################# #rmodel now provides mas5 background correction and constant median normalization in addition to building the rmodel batch object myrmodel<-rmodel(mydata, sample.a=c(1,2,3), sample.b=c(4,5,6), label.a="gv", label.b="m2", mas5calls=3, masChips=c(1,2,3,4,5,6), nullmodel="within.grp", mode="sample") myrmodel<-buildOdds(myrmodel,fd.rate=0.1,useRdiff=TRUE,min.set=3,n=30000) myrmodel<-scoreSegs(myrmodel) genomeTracks(myrmodel) save.image(file=paste(myrmodel@title,".myrmodel.Rdata",sep="")) ############################################################################################# #rmodel now provides mas5 background correction and constant median normalization in addition to building the rmodel batch object myrmodel<-rmodel(mydata, sample.a=c(1,2,3), sample.b=c(7,8,9), label.a="gv", label.b="m2pa", mas5calls=3, masChips=c(1,2,3,7,8,9), nullmodel="within.grp", mode="sample") myrmodel<-buildOdds(myrmodel,fd.rate=0.1,useRdiff=TRUE,min.set=3,n=30000) myrmodel<-scoreSegs(myrmodel) genomeTracks(myrmodel) save.image(file=paste(myrmodel@title,".myrmodel.Rdata",sep="")) ############################################################################################# #rmodel now provides mas5 background correction and constant median normalization in addition to building the rmodel batch object myrmodel<-rmodel(mydata, sample.a=c(7,8,9), sample.b=c(10,11,12), label.a="m2pa", label.b="dko", mas5calls=3, masChips=c(7,8,9,10,11,12), nullmodel="within.grp", mode="sample") myrmodel<-buildOdds(myrmodel,fd.rate=0.1,useRdiff=TRUE,min.set=3,n=30000) myrmodel<-scoreSegs(myrmodel) genomeTracks(myrmodel) save.image(file=paste(myrmodel@title,".myrmodel.Rdata",sep=""))