Priyam Singh, PhD

For my PhD dissertation, I collaborated to develop a novel microarray prove-level algorithm and applied it to a mouse leukemia model to discriminate histologically similar subtypes of mouse lymphoma samples based on differences in the 3'-untranslated region. I demonstrated the critical importance of measuring changes in the distribution of alternative isoforms, as well as overall transcript levels and provided a possible link between genotoxic stress and alternative 3'-processing. I performed extensive DNA/RNA sequence analysis to determine that difference in isoforms differing at the 3'-end arise from both systematic selection of 3'-processing sites and differential degradation instigated by miRNA activity.

Some of my research interests involve investigating role of miRNA activity in cancer system, developing diagnostic algorithms using microarray or next-generation deep sequencing for early diagnosis of cancer, developing statistical methods to unravel deep-sequencing data and exploring gene-protein, protein-protein interactions of signalling pathways in cancer.